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Yes

Stanley Tucci as Leonardo da Vinci (voice)
Rich Dietl as Commander / Advisor Smith (voice)
Jonathan Holmes as Percy (voice)
Tomokazu Sugita as Abigail
Bill Hader as The Voice (voice)
Shizuka Ishigami as Laufen
Oota Volcano as Genji Yamada Asaemon
Saya Aizawa as Ayano Kimishima
Yuhko Kaida as Amane Oguro
Steven Tyler as Nim Galuu (voice)
Michael Palin as Ernie Clicker (voice)
Marina Inoue as アンダーテイカー・リーダー / Under Taker
Stephen Kearin as Technician Bee (voice)
David L. Lander as Bird (voice)
Daiki Yamashita as Yamada Asaemon Senta
Chantal Strand as Rose (voice)
Michael McKean as Dr. Bartholomew Wolper (voice)
Stephen Colbert as Paul Peterson (voice)
Jeffrey Tambor as Carl Murphy (voice)
Britt Irvin as Mila (voice)
Hiro Shimono as Marcus Lithos
Kisho Taniyama as Wirbel
Mayumi Iizuka as Tsuneko Tani (voice)
Kentaro Tone as Weak Kingsley

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