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Yes

Art Gilmore as Narrator (voice)
Lily Tomlin as Professor Valerie Felicity Frizzle, Ph.D.
Daran Norris as Additional Voices (voice)
Hirotaka Suzuoki as Oda Tatsumasa
Keith Szarabajka as Dr. Kyne (voice)
Kristen Wiig as Katherine (voice)
Ian James Corlett as Mr. Mint / Snow Beaver (voice)
Chad Stuart as British Announcer (voice)
Gianfranco Mauri as Gastone (voice)
Natsuki Hanae as Hideyoshi Toyotomi
Megan Follows as Clara (voice)
Jesus Adrian SLU as Mexico Countryballs
Jon Lovitz as Tom Baltezor (voice)
Saeko Shimazu as Megumi Ayase (voice)
George Lopez as Zook (voice)
Claire Guyot as Les Scies, Clarinette et Clarinette Basse (voice)
Desiree Goyette as Woman at Door (voice)
Mai Fuchigami as 滝本 かよ / Kayo Takimoto
Shigeru Chiba as Dentro
Erik Stappenbeck as Biberbruder Henry (voice)
Joan Allen as Narrator (voice)
India Fisher as Charley Pollard
Breckin Meyer as Superman / Bizarro (voice)

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